We are sharing our protocols
CULTURE MEDIA
Artificial Seawater (ASW)
ASW, adapted for Ruegeria pomeroyi
Contamination plate
PROTEOMICS
Cell extract and exoproteome
TCA precipitation
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Protein gel
GENETIC ENGINEERING
Design for Gibson assembly
Conjugation in mineral marine media
Generation of mutants in Synechococcus (see Note 1 of the pdf)